Figure 3.

Flow cytometric analysis of bone marrow cells from nontransgenic and dTg (Ig-tTA/tet-μ) rag-2^−/− mice. Bone marrow cells from 6-wk-old rag-2^−/− and dTg rag-2^−/− mice that had received drinking water with (200 μg/ml) and without tetracycline were stained with fluorochrome-conjugated antibodies indicated adjacent to the axes. Cells falling into the lymphoid gate were analyzed. The numbers indicate percentages of cells contained within each quadrant. Similar results were obtained in experiments with five rag-2^−/− mice and three dTg rag-2^−/− mice. The percentage of CD25⁺ cells among all B220⁺ cells in dTg rag-2^−/− mice that did not receive tetracycline varied between 45 and 81% in four different mice analyzed.