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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cancer Res. 2010 Aug 24;70(17):6697–6703. doi: 10.1158/0008-5472.CAN-10-1271

Figure 4.

Figure 4

Knockdown of membrane-localized NHERF1 increases proliferation and Akt activation. A. The % depletion (dep.) is indicated for knockdown NHERF1 levels normalized to GAPDH. The proliferation of vector-control and NHERF1-depelted cells were performed with two different NHERF1 shRNAs (sh4 shown) and repeated three times with similar results. Values are means±SEM from triplicates; **=p<0.01. B. Time course stimulation of NHERF1-depleted and control cells with 10% FBS. Mean±SEM P-Akt/Akt values from two experiments: *=(0.0047<p<0.032). C. Time course stimulation of PTEN-depleted and control cells as in (B). Mean±SEM P-Akt/Akt values calculated from three experiments: *=(0.0016<p<0.016). D. Model depicting NHERF1 regulation of PTEN in normal and transformed cells. The dotted line indicates less efficient Akt suppression by PTEN in the absence of membrane NHERF1.