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. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: J Mol Cell Cardiol. 2010 Jul 23;49(4):617–624. doi: 10.1016/j.yjmcc.2010.07.011

Simulated ECC Gain under varying degrees of LCC and RyR phosphorylation. To load the SR with Ca²⁺, membrane potential was clamped to 0 mV for 200 ms from a holding potential of −70 mV repeatedly at 0.5 Hz for 10 s. Membrane potential was then clamped to a variable test potential for 200 ms. ECC gain is calculated using peak LCC and RyR flux obtained during the test potential. The simulation was performed 20 times at each membrane potential. Results are presented as mean ± S.E.M. (A) ECC gain in the absence of LCC phosphorylation, with varying degree of RyR phosphorylation. The maximum RyR phosphorylation rate was altered to 25% or 1000% of normal to obtain 4.4% or 66% RyR phosphorylation, respectively. (B) Same protocol as panel A where prior to the test pulse, Ca_SR was set to 0.75 mM and Ca_cyt was set to 75 nM. (C) ECC gain in the absence of RyR phosphorylation, with varying degree of LCC phosphorylation. The maximum LCC phosphorylation rate was altered to 50% or 150% of normal to obtain 0.6% or 6.4% LCC phosphorylation, respectively. (D) Same protocol as panel C where prior to the test pulse, Ca_SR was set to 0.75 mM and Ca_cyt was set to 75 nM.

Simulated ECC Gain under varying degrees of LCC and RyR phosphorylation. To load the SR with Ca²⁺, membrane potential was clamped to 0 mV for 200 ms from a holding potential of −70 mV repeatedly at 0.5 Hz for 10 s. Membrane potential was then clamped to a variable test potential for 200 ms. ECC gain is calculated using peak LCC and RyR flux obtained during the test potential. The simulation was performed 20 times at each membrane potential. Results are presented as mean ± S.E.M. (A) ECC gain in the absence of LCC phosphorylation, with varying degree of RyR phosphorylation. The maximum RyR phosphorylation rate was altered to 25% or 1000% of normal to obtain 4.4% or 66% RyR phosphorylation, respectively. (B) Same protocol as panel A where prior to the test pulse, Ca_SR was set to 0.75 mM and Ca_cyt was set to 75 nM. (C) ECC gain in the absence of RyR phosphorylation, with varying degree of LCC phosphorylation. The maximum LCC phosphorylation rate was altered to 50% or 150% of normal to obtain 0.6% or 6.4% LCC phosphorylation, respectively. (D) Same protocol as panel C where prior to the test pulse, Ca_SR was set to 0.75 mM and Ca_cyt was set to 75 nM.

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