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Analysis of the interaction of α2-antiplasmin with fibrinogen and fibrin by ELISA. Increasing concentrations of biotinylated α2-AP were added to surface-adsorbed fibrinogen (empty circles) or fibrin (filled circles). Bound biotinylated α2-AP was detected with avidin conjugated to alkaline phosphatase as described in Experimental Procedures. The curves represent the best fit of the data to eq. 1. All results are means ± the standard deviation of duplicate determinations.
