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. Author manuscript; available in PMC: 2011 Oct 10.
Published in final edited form as: Virology. 2010 Aug 1;406(1):103–116. doi: 10.1016/j.virol.2010.07.012

Figure 7. Signal induction during KSHV de novo infection of THP-1 cells.

Figure 7

THP-1 cells, serum starved for 24 h, were left uninfected or infected with KSHV (20 DNA copies/cell) for the indicated time. Proteins prepared (15μg) from the infected and uninfected THP-1 were resolved by SDS-PAGE and transferred onto nitrocellulose membranes. Phosphorylated and total (A) FAK, (B) Src, (C) PI3K, (D) NF-κB and (E) ERK1/2 proteins were detected with the respective antibodies. The fold induction of phosphorylation was calculated based on the ratio of phosphorylated/total at each time point and values represent fold phosphorylation compared uninfected. HS pretreated KSHV (1 h at 37°C) was used as control. (F) Effect of tyrphostin and genistein on KSHV internalization. Untreated and THP-1 cells preincubated with Tyrphostin and Genistein (1 h at 37° C) were infected with KSHV. At 2 h p.i., mock treated and treated cells were washed and trypsinized to remove uninternalized viruses. Internalized KSHV copy number was determined as described in figure legend 1C/D. The data are represented as the percent inhibition of KSHV DNA internalization in comparison with untreated THP-1 cells.