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. Author manuscript; available in PMC: 2011 Oct 10.
Published in final edited form as: Virology. 2010 Jul 27;406(1):80–87. doi: 10.1016/j.virol.2010.07.008

Figure 5. Administration of PIKA induced production of cytokines in the lungs.

Figure 5

Groups of three mice each were given 100 μg of PIKA i.n. in PBS and were sacrificed at the indicated time. The lungs were stored at −80°C till all samples were collected and homogenized in 1 mL of RPMI-1640 media. (A–C) To measure the level of different cytokines/ chemokines in the samples, 50 μL of the clarified samples were tested in duplicate using the Bio-plex Protein Array system. (D) For IFN-β, 100 μL of the clarified samples were tested using an ELISA kit. The bars and error bars represent the mean and standard error of the groups.