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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cancer Res. 2010 Aug 24;70(17):6837–6848. doi: 10.1158/0008-5472.CAN-10-1075

Figure 5. Active transcription is required for inhibition of HIF-1α mRNA expression by AF.

Figure 5

A–B. MCF-7 were cultured under normoxia or hypoxia for 16 hours in the absence or presence of AF (0.25 μM). HIF-1α mRNA (A) and HIF-1α pre-mRNA (B) expression was analyzed. ** = p<0.005, * = p<0.01, relative to medium. C–D. MCF-7 were cultured under normoxia or hypoxia for 16 hours in the absence or presence of AF (0.25 μM) and/or actinomycin D (5μg/ml). HIF-1α mRNA (C) and HIF-1α pre-mRNA (D) expression was assessed. *** = p<0.001, ** = p<0.005, relative to medium.