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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cytotherapy. 2009;11(7):912–922. doi: 10.3109/14653240903136987

Figure 4. The phenotypic profiles of fluMP, Melan-A and NY-ESO-1 tetramer-positive CD3+CD8+ cells are not affected by different cytokine conditions in culture medium.

Figure 4

Multiparameter flow cytometry was performed to analyze the phenotype of tetramer-positive CD3+CD8+ cells after a 10-day in vitro culture with MelanA peptide stimulation using the markers CCR7, CD45RA, CD27 and CD28 under three different cytokine conditions in the growth medium: IL-2 + IL-7, IL-2 + IL-15 and IL-2 + IL-7 + IL-15. Figure 4A represents a bar chart of the different phenotypic combinations under the three different cytokine conditions. Figure 4B is a pie chart representing the different phenotypic combinations for each cytokine condition.