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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cytotherapy. 2009;11(7):912–922. doi: 10.3109/14653240903136987

Figure 5. Longer culture time leads to expansion of the CD8+ CCR7-CD45RA- subset.

Figure 5

Multiparameter flow cytometry with the markers CCR7 and CD45RA was performed to analyze the phenotype of tetramer-positive CD3+CD8+ cells after 10 and 14 days of in vitro culture. (A) A representative dot plot indicates an increase in the population of CCR7-CD45RA- NY-ESO-1 tetramer-positive CD3+CD8+ T cells between Days 10 and 14 of in vitro culture. (B) This chart represents an average of CCR7/CD45RA subsets for fluMP, Melan-A and NY-ESO-1 tetramer-positive CD3+CD8+ T cells at Day 0, 10 and 14 of in vitro culture. The proportion of CCR7-CD45RA- cells increases with the length of culture time.