Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cancer Res. 2010 Aug 16;70(17):6968–6977. doi: 10.1158/0008-5472.CAN-09-4474

Figure 3.

Figure 3

GFP is silenced in YB5, and heterogeneously reactivated by DAC. (a) The expression of GFP mRNA in SW48, YB5 and gradient DAC treated YB5 cells. Four days treatment of gradient amount of DAC restored GFP expression in a dose-dependent manner. YB11 clone, positive control. (b) Fluorescent plots of SW48, YB5, YB11 and DAC reactivated YB5 cells (100nM treatment as an example). (c) FACS analysis of GFP positivity of YB5 cells treated with increasing amounts of DAC described in Figure 3a. The cutoff line of GFP positivity is shown as the horizontal line in Figure 3b. (d) Correlation of GFP% obtained by FACS analysis to mRNA expression obtained by real-time PCR. The correlation was significant (P < 0.05) determined by Pearson test.