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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cell Res. 2010 Jul 13;20(9):1012–1022. doi: 10.1038/cr.2010.93

Figure 5.

Figure 5

Spontaneous ploidy duplication and stable DNA content of hypertetraploid cells during in vivo metastasis progression. (A) Two representative lung pieces with multiple metastasis nodules formed after intravenous injection of LM2 cells. Tumor #1 represents predominantly maintained hyperdiploidy of tumor cells in the nodules, while tumor #2 represents partially converted ploidy from hyperdiploidy to hypertetraploidy in the nodules One out of 3 lung pieces contained detectable hypertetraploid nodules. Inserts show the enlarged areas of the DNA content profiles. (B) Two representative bone marrow flushes with metastases formed after intracardiac injection of BM1 cells. Tumor #1 represents fully maintained hyperdiploidy of tumor cells, while tumor #2 represents almost completely converted ploidy from hyperdiploidy to hypertetraploidy (accounting for 18.2% of 11 tested bone samples). (C) Fully maintained hypertetraploidy of lung metastases formed by LM2-hyper (accounting for 100% of 4 tested lung samples). (D) Fully maintained hypertetraploidy of bone metastases formed by BM1-hyper (accounting for 100% of 2 tested bone samples). Legend at the bottom shows signs representing different ploidy peaks of human or mouse origin. N=haploidy of MDA-MB-231.