. Author manuscript; available in PMC: 2011 Sep 7.

Published in final edited form as: Biochemistry. 2010 Sep 7;49(35):7704–7708. doi: 10.1021/bi101118g

Table 1.

Kinetic Parameters for Protein-Catalyzed Reactions of [1-¹³C]-GA in D₂O to form [1-¹³C, 2-²H]-GA and [1-¹³C, 2,2-di-²H]-GA at 25 °C and pD 7.0.

Catalyst	(k_E)_obsd (M⁻¹ s⁻¹) ^a	f_P^b	(k_E)_P (M⁻¹ s⁻¹) ^c
Wildtype Chicken TIM ^d	0.19	≈ 0.2	≈ 0.04
K12G Chicken TIM ^e	0.11 ± 0.005	0.56 ± 0.05	0.06 ± 0.006
BSA ^f	0.49 ± 0.02	0.51 ± 0.03	0.25 ± 0.02

	0.0065 M⁻¹ s⁻¹ ^g

The observed second-order rate constant for the protein-catalyzed reactions of [1-¹³C]-GA. The second-order rate constants for catalysis by TIM are calculated for one monomer of this dimeric enzyme. The quoted errors are the standard deviation of linear correlations of reaction progress against time.

The fractional yield of the products from the protein-catalyzed reactions of [1-¹³C]-GA. This is the average of the product yields determined at 3–5 different reaction times.

The second-order rate constant for the protein-catalyzed deuterium exchange reactions of [1-¹³C]-GA: (k_E)_P = f_P(k_E)_obs.

Data from reference (12)

Data from reference (13).

This work.

The second-order rate constant for general base-catalyzed deprotonation of glyceraldehyde by 3-quinuclidinone (22).