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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cancer Res. 2010 Jul 19;70(17):6704–6714. doi: 10.1158/0008-5472.CAN-10-0460

Figure 5.

Figure 5

Combined inhibition of MEK and mTOR in MDA-MB231 cells. (A and B) Protein levels were detected in unstimulated cells in the absence of inhibition (column 1) and after 1 hour of incubation with the MEK or/and mTOR inhibitors (columns 2-4) and in cells stimulated with IGF-1 in the absence of inhibition (column 5) and after 1 hour of incubation with the MEK or/and mTOR inhibitors (columns 6-8). Cells were stimulated with 75 ng/mL IGF-1 for 5 minutes (A) or 60 minutes (B). (C and D) Density of the bands after normalization with respect to actin.