Table 1.
Percentage of MPS, MPO, DMTP and/or DMP in cell cytosol after exposure of cells to 1 μM of OP (MPS or MPO)1
Incubation time (h) | Percentage of MPS, MPO, DMTP and/or DMP in cell cytosol (per 106 cells)2 |
Relative Ratio’s |
||||
---|---|---|---|---|---|---|
MPS | MPO | DMP and/or DMTP | MPS | MPO | DMP and/or DMTP | |
14C-MPS | ||||||
24 | 0.036 ± 0.001 | 0.026 ± 0.002 | 0.017 ± 0.001 | 45.3 ± 1.5 | 33.0 ± 0.9 | 21.7 ± 0.6 |
48 | 0.054 ± 0.006 | 0.045 ± 0.006 | 0.020 ± 0.004 | 45.8 ± 2.0 | 37.9 ± 1.7 | 16.3 ± 1.1 |
72 | 0.043 ± 0.004 | 0.037 ± 0.003 | 0.018 ± 0.002 | 43.5 ± 1.8 | 37.9 ± 2.3 | 18.7 ± 0.6 |
14C-MPO | ||||||
24 | - | 0.014 ± 0.007 | 0.106 ± 0.014 | - | 10.9 ± 4.5 | 89.1 ± 4.5 |
48 | - | 0.037 ± 0.005 | 0.139 ± 0.008 | - | 21.2 ± 2.3 | 78.8 ± 2.3 |
72 | - | 0.025 ± 0.002 | 0.084 ± 0.003 | - | 22.5 ± 0.7 | 77.5 ± 0.7 |
Ratios of OP and their degradation products were determined by preparative TLC analysis;
Data represents mean ± SEM (n = 3).