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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cancer Res. 2010 Aug 24;70(17):6870–6879. doi: 10.1158/0008-5472.CAN-10-1378

Figure 5.

Figure 5

The treatment schedule of DAPT and TMZ affects neurosphere recovery. A, Schematic of the recovery assay treatment schedules. TMZ (diamond) and DAPT (inverted triangle) treatments were either administered with DAPT 24 hours prior to TMZ (PRE-treat), simultaneously with TMZ (CO-treat), or 24 hours after TMZ (POST-treat). B, U87NS cultures and C, GS7-2 cultures were treated with different DAPT treatment schedules and recovery was analyzed. Initial neurospheres were counted on day 7 or 10 (mean ± SD), recovery neurospheres were counted on day 14 or 20, and secondary neurospheres were counted on day 21 or 30. Secondary neurosphere formation was inhibited only in cultures treated with the POST-treat schedule. The t-test was used to calculate statistical significance. *=P<0.001.