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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Toxicol Lett. 2010 Jun 9;197(3):219–226. doi: 10.1016/j.toxlet.2010.06.003

Fig. 5. The repression of wild type and 15 natural human PXR variants mediated by IL-6.

Fig. 5

HepG2 cells were transiently transfected with a mixture containing 50 ng of CYP3A4-DP-Luc, 50 ng wide type or nature human PXR variant, along with 5ng of the Null-Renilla reniformis luciferase plasmid. The transfected cells were treated with 10ng/ml IL-6 or the same volume of PBS for 24 h. Luciferase activities were determined as described in the above. To determine whether the expression is altered with certain variants, cell lysates(8µg) were analyzed by Western blotting(Anti-hPXR) for the expression level(bottom). Three independent experiments were performed, and the data were expressed as mean ± S.D.