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mRNAs of CD40 isoforms from indicated cells and organs were analyzed by RT-PCR using P1 and P2 primers (Fig. 1A). HPRT mRNA was analyzed also by RT-PCR as control. (A) RNA was isolated from organs and cells from normal mice. If required, cells were activated by phorbol 12-myristate 13-acetate, ConA, LPS, or IL-4 + anti-CD40 antibody FGK 45. (B) RNA was isolated from the SOCS-1 transfectant. If required, cells were activated by IFN-γ and LPS.
