Table 1.

Summary of anti- apoptotic and autophagic cell engineering strategies carried out in cell lines used for the production of recombinant proteins

Gene symbol	Gene name	Cell line	Author
Apoptosis: over-expression
Aven	Apoptosis, caspase activation inhibitor	CHO	Figueroa et al. (2007)
Bcl-2	B-cell/lymphoma 2	CHO, hybridoma, NS0, BHK, BL	Tey et al. (2000a), Itoh et al. (1995), Simpson et al. (1997) and Singh et al (1996) etc.
Bcl-xl	Bcl-2-like 1	CHO, hybridoma, BHK	Meents et al. (2002), Mastrangelo et al. (2000) and Figueroa et al. (2004)
Bag-1	Bcl-2-associated athanogene 1	Hybridoma	Terada et al. (1997)
Bhrf1	BCL2-interacting killer (apoptosis-inducing)	Hybridom	Juanola et al. (2009)
CrmA	Caspase 1, apoptosis-related cysteine protease inhibitor	CHO, HEK293	Sauerwald et al. (2003)
E1b-19k	Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3	CHO, NS0, BHK	Figueroa et al. (2007)
Fadd	Fas (TNFRSF6)-associated via death domain	CHO	Wong et al. (2006)
Faim	Fas apoptotic inhibitory molecule	CHO	Wong et al. (2006)
Hsp70	Heat shock 70kDa protein	NS0	Lasunskaia et al. (2003)
Htert	Telomerase reverse transcriptase	CHO	Crea et al. (2006)
Mcl-1	Myeloid cell leukemia sequence 1 (Bcl2-related)	CHO	Major and Betenbaugh (2009)
Mdm2	Mdm2 p53 binding protein homolog	CHO	Arden et al. (2006)
Xiap	X-linked inhibitor of apoptosis	CHO, HEK293	Sauerwald et al. (2003)
Apoptosis: knock-out (small interference RNA)
Alg2	Asparagine-linked glycosylation protein 2	CHO	Wong et al. (2006)
Bak	Bcl-2-antagonist/killer 1	CHO	Lim et al. (2006) and Cost et al. (2009)
Bax	Bcl-2-associated X protein	CHO	Lim et al. (2006) and Cost et al. (2009)
Caspase 3, 7	Caspases	CHO	Sung et al. (2005)
Caspase 8, 9	Caspases	CHO	Yun et al. (2007)
Requiem	Apoptosis response zinc finger	CHO	Wong et al. (2006)
Autophagy: over-expression
ca-Akt	Protein kinase B	CHO	Hwang and Lee (2009)
Bcl-xl	Bcl-2-like 1	CHO	Kim et al. (2009)