Table 3.
Accuracy of taxonomic classification of 219 foregut species using 350-bp sequences
Amplicon |
% accuracy compared to 900-bp amplicon/full length |
||||
Phylum | Class | Order | Family | Genus | |
Forward reads | |||||
A | 97.7/97.7 | 96.3/95.9 | 95.9/95.4 | 93.2/94.5 | 87.7/86.8 |
B | 99.1/99.1 | 98.2/96.8 | 97.7/96.3 | 97.3/95.9 | 91.8/89.0 |
C | 99.5/99.5 | 98.2/96.8 | 97.7/96.8 | 97.3/95.9 | 90.4/88.1 |
D | 98.6/99.1 | 98.6/98.2 | 97.3/97.3 | 95.9/96.3 | 88.1/86.8 |
E | 98.2/98.6 | 97.7/98.2 | 95.4/95.9 | 92.2/93.6 | 85.4/84.9 |
F | 97.7/98.2 | 96.3/97.3 | 95.0/95.4 | 91.8/93.6 | 83.6/84.5 |
Reverse reads | |||||
A’ | 98.6/98.6 | 97.3/97.7 | 97.3/97.7 | 95.0/96.3 | 90.0/90.9 |
B’ | 99.5/99.5 | 98.2/96.8 | 97.7/96.8 | 97.7/96.3 | 93.6/91.8 |
C’ | 99.5/99.5 | 98.2/96.8 | 97.7/96.8 | 97.3/95.9 | 91.3/90.0 |
D’ | 99.5/100 | 98.6/99.5 | 96.8/98.2 | 94.5/96.3 | 87.2/89.5 |
E’ | 98.2/98.6 | 96.8/98.2 | 94.5/96.3 | 91.3/94.1 | 83.6/87.2 |
F’ | 98.2/98.6 | 96.8/98.2 | 95.0/96.4 | 92.2/95.0 | 82.6/85.8 |