Figure 2.
Example of (ORF YOR054C DNTAG) sequence where Pyrosequencing confirms the putative sequence, which is not confirmed by Sanger dideoxy sequencing. (a) The putative sequence, (b) pyrogram from Pyrosequencing. The shadowed rectangle marks the region with the correct tag sequence (20 bases). The Pyrogram sequence agrees with putative sequence, (c) electropherogram from Sanger dideoxy sequencing. Arrow indicates where pyrogram agrees with the putative sequence, but not with the Sanger-derived sequence. The Sanger dideoxy sequencing result has a substitution of C to G (q = 39) at the position of the tag.