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. 2010 Sep 1;24(17):1903–1913. doi: 10.1101/gad.1935910

Figure 2.

Figure 2.

Deletion of LYS20 and LYS21 suppresses htz1Δ DNA damage sensitivity. (A) Resistance to camptothecin is dependent on ESA1. Strains were assayed as in Figure 1. The YPD-based camptothecin (CPT) plate contains 40 μg/mL camptothecin in DMSO. (B) Deletion of LYS20 and LYS21 suppresses htz1Δ DNA damage sensitivity. All plates were incubated at 33°C. All plates are YPD-based. The camptothecin (CPT) plates contain 20 μg/mL camptothecin in DMSO, and were incubated 5 d longer than the growth control. (C) Suppression is dependent on ESA1. Independent strain isolates were assayed as in Figure 1. Plates were incubated at 30°C. All plates are YPD-based. The camptothecin (CPT) plate contains 20 μg/mL camptothecin in DMSO. Vertical bars indicate platings of two independent triple mutants. (D) Suppression is evident with HU-induced damage. Strains were assayed as before on YPD or synthetic complete as growth control, or on 0.1 M HU. The HU plate was incubated an additional 3 d in the top panel, and an additional 5 d in the bottom panel. Plates were incubated at 30°C. Vertical bars indicate platings of two independent triple mutants.