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. 2010 Sep 1;24(17):1903–1913. doi: 10.1101/gad.1935910

Figure 3.

Figure 3.

Rad53 phosphorylation status during DNA damage and suppression. (A) The Rad53 phosphorylation shift upon HU-induced DNA damage is not significantly affected by overexpression of LYS20. Extracts were prepared for cells with the indicated genotypes. Immunoblots with anti-Rad53 antibody are shown. In samples treated with HU, the proportion of phosphorylated Rad53 was quantified, and is indicated as a percentage of total Rad53. (B) The htz1Δ mutants had reduced Rad53 phosphorylation upon DNA damage. (C) The htz1Δ mutants had reduced Rad53 phosphorylation upon DNA damage. This defect was suppressed by deletion of LYS20 and LYS21.