Figure 2.

Nab2 and Yra1 are part of the same mRNA export pathway. (A) FISH analysis of GAL1 mRNA in the indicated cells induced with galactose for 30 min at 30°C. DAPI staining or FISH/DAPI overlays are shown below each panel to localize the RNA signal with respect to the nucleus. (B) Δyra1 is bypassed by overexpressing NAB2 or MEX67. The Yra1 shuffle strain transformed with the indicated plasmids was spotted as 10-fold serial dilutions onto 5-FOA to select against the wild-type pURA3-YRA1 plasmid, or onto a selective plate to control for cell number (last two dilutions only). (2μ) High-copy plasmids.