Figure 3.

Dependence of CRU and CFC expansion in vitro on the concentration of H-IL-6 present. Sca-1⁺lin⁻ mouse BM cells were cultured in serum-free medium containing SF (50 ng/ml) and FL (100 ng/ml) and varying concentrations of H-IL-6. The fold increase in CRU and CFC (±SEM, n = 2–4) was calculated by dividing the number of these cells detected after 10 days by the corresponding numbers present in the number of Sca-1⁺lin⁻ cells used to initiate each culture.