Figure 4.

Similar dose effects of different gp130-activating cytokines on CRU and CFC expansion. Sca-1⁺lin⁻ mouse BM cells were cultured in serum-free medium containing SF (50 ng/ml) and FL (100 ng/ml) and various gp130-activating cytokines at the following concentrations: IL-6 at 20 ng/ml, sIL-6R at 400 ng/ml, IL-11 at 100 ng/ml, H-IL-6 at 40 ng/ml when used alone, and H-IL-6 at 10 ng/ml when used in the four-factor combination. The fold increase in CRU and CFC (±SEM, n = 4–8) was calculated by dividing the number of these cells detected after 11 days by the corresponding numbers present in the number of Sca-1⁺lin⁻ cells used to initiate each culture.