Figure 6.

Presence of gp130-activating cytokines has no effect on the survival or initial mitogenesis c-kit⁺Sca-1⁺lin⁻ cells also stimulated by SF and FL. Clone size distribution (bars) and cumulative frequency of clones (symbols) generated in 10 days by single PI⁻ c-kit⁺Sca-1⁺lin⁻ mouse BM cells incubated in serum-free medium supplemented with SF (50 ng/ml) and FL (100 ng/ml) in combination with (Top) IL-6 at 50 ng/ml (closed bars and ▴) or no other cytokine (open bars and ○, n = 95 wells); (Bottom) H-IL-6 at 40 ng/ml (closed bars and ■, n = 96 wells) or no other cytokine (open bars and ○, n = 96 wells).