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. 2010 Apr-Jun;4(2):94–104. doi: 10.4161/pri.4.2.12622

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© 2010 Landes Bioscience

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Detection of SH aPrP (90-231) by ELISA with antisera from prion immunized Prnp^0/0/Balbc/J, but not wt Balbc/J mice. Antisera from Prnp^0/0/Balbc/J mice detected SH aPrP (90-321) at dilutions >1:30 K by direct binding ELISA; whereas antisera from immunized wt Balbc/J mice failed to detect SH aPrP (90-231) at any dilution (A). Antisera from prion immunized Prnp^0/0/Balbc/J, but not wt mice, detected SH aPrP (90-231), Syrian hamster brain PrP^C (Ha PrP^Sc DRM; no PK) and PrP^Sc (Ha PrP^Sc DRM; +PK) by Western blot (B). Detection of PK-resistant PrP^Sc was observed with antisera diluted >25 K from two representative Prnp^0/0/Balbc/J (#1–2) and wt Balbc/J (#3–4) mice immunized with purified prion antigen. RLU = relative light units.