Figure 8.

Antisera from Prnp^0/0/Balbc/J mice immunized with purified PrP^Sc binds Syrian hamster prion proteins. Western blot comparing binding of antisera from a prion imunized Prnp^0/0/Balbc/J to normal and infectious hamster brain homogenate (30 µg/lane), recSH aPrP(90-231); 100 ng/lane) and normal and infectious hamster brain DRM (10 µg/lane) preparations (top part). Antisera detected PK-sensitive PrP^C, recSH aPrP(90-231) and PK-insensitive PrP^Sc in brain homogenates and DRM preparations; no other protein bands were observed. A comparative western blot showed similar binding of the monoclonal anti-prion antibody IPC1 to brain homogenate, recombinant PrP and DRM preparations (bottom part) as antisera from Prnp^0/0/Balbc/J mice. PK = proteinase-K treatment (+). Protein normalization by BCA.