Table 2.
hAChE | OP | OXIME | Relative Reactivation Rate |
---|---|---|---|
wt 1 | Paraoxon | 2PAM | 1 |
D134H | 6 | ||
D134H_E202Q | 0.1 | ||
D134H_F338A | 1 | ||
wt 2 | HI6 | 1 | |
D134H | 3 | ||
wt 3 | SP CHMPTCh | 2PAM | 1 |
D134H | 2 | ||
wt 4 | HI6 | 1 | |
D134H | 0.3 | ||
D134H_E202Q | 0.02 | ||
D134H_F338A | 0.002 | ||
wt 5 | VX analogue | 2PAM | 1 |
D134H | 1 | ||
wt 6 | RP IPMPTCh | 2PAM | 1 |
D134H | 1 |
The second order rate constants for reactivation of OP inhibited wt hAChE by oximes were:
120 M-1min-1 (paraoxon inhibited hAChE reactivated by 2PAM)
45 M-1min-1 (paraoxon inhibited wt hAChE reactivated by HI6)
275 M-1min-1 (SPCHMPTCh inhibited wt hAChE reactivated by 2PAM)
13000 M-1min-1(SPCHMPTCh inhibited wt hAChE reactivated by HI6)
490 M-1min-1(VX inhibited wt hAChE reactivated by 2PAM)
270 M-1min-1(RPIPMPTCh inhibited wt hAChE reactivated by 2PAM).
Reactivation of cyclosarin and soman inhibited hAChE was not measured.