Table 4.

Few CTb-labeled corneal afferents contained both CGRP and VGluT2 immunoreactivity ^*

Region	CGRP-ir + VGluT2-ir	CGRP-ir	VGluT2-ir	Neither
Caudal – Vc/C1	2 (1%)	5 (3%)	7 (4%)	161 (92%)
Rostral – Vi/Vc	7 (2%)	32 (9%)	39 (11%)	272 (78%)

^*Each column in the table indicates the raw number of varicosities in that category for each region. Percentages indicate the breakdown of CTb-labeled varicosities within that region. Total number of CTb-labeled varicosities were 175 (caudal) and 350 (rostral) from 7 animals. There was a higher proportion of CGRP-containing to total CTb-labeled varicosities rostrally than caudally (z = 2.359, P = 0.018). We found the same was true for VGluT2-containing varicosities (z = 2.523, P = 0.018). There was no difference in the proportion of CTb-labeled varicosities that contained both CGRP and VGluT2 in rostral Vi/Vc as compared to caudal Vc/C1 (z = 0.482; P = 0.630).