Figure 5.

Kisspeptin induces an inward current in POMC cells. The inward current was recorded at a holding potential of −60 mV with TTX (0.5 μm) in the bath. A, Kisspeptin (1 μm) evoked an inward current in a POMC neuron in normal ACSF. B, The inward current induced by kisspeptin (1 μm) in nominal zero-Ca²⁺/10 mm Mg²⁺ ACSF solution. C, The kisspeptin induced inward current in a POMC neuron was blocked with BAPTA (10 mm) in the pipette solution. D, Inward current induced by kisspeptin on a POMC cell in an ACSF solution with Na⁺ replaced by Li⁺. E, The kisspeptin induced current on a POMC cell in the presence of NCX blocker KB-R7943 (60 μm). F, Kisspeptin induced current in a POMC cell in the presence of a more selective NCX blocker SN-6 (10 μm). G, Kisspeptin (1 μm) induced a small inward current in the presence of SKF96365 (10 μm). H, Bar graph showing the mean amplitude of kisspeptin induced current in POMC cells during different recording conditions. *p < 0.05; **p < 0.01; group t test. Error bars indicate SEM.