Figure 6.

Kisspeptin-evoked current is attenuated by Li⁺ and KB-R7943. The current traces were evoked by voltage ramps from −130 to 0 mV at a holding potential of −60 mV. TTX (0.5 μm), AP5 (50 μm), CNQX (10 μm), BIC (30 μm), and Cd²⁺ (200 μm) were added in the bath solution. A, Recordings performed with a normal ACSF bath perfusion and a KMeSO₄ pipette solution. Top, Traces showing the current responses to the voltage ramp in POMC cells before (Cont) and during kisspeptin (Kiss) (1 μm). Bottom, The Kiss-induced current (subtraction of Cont from Kiss). B, Cont and Kiss (top) and Kiss-induced current (bottom) recorded with BaCl₂ (0.3 mm) in the bath perfusion and a CsMeSO₄ pipette solution. C, Kiss-induced current recorded using a CsMeSO₄ pipette solution in modified ACSF with Na⁺ replaced by Li⁺. D, Kiss-induced current recorded using a CsMeSO₄ pipette solution after pretreatment with NCX blocker KB-R7943 (60 μm). E, Bar graph showing the amplitude of Kiss-induced current at −125 mV under different recording conditions. *p < 0.05; **p < 0.01 vs normal ACSF; group t test. Error bars indicate SEM.