Figure 7.

Group I metabotropic glutamate receptor agonist DHPG and kisspeptin both excite the same POMC cells. A, DHPG (10 μm) stimulated the firing of a POMC cell and depolarized the cell, and the same cell was also excited by kisspeptin (1 μm) applied later. B, DHPG (50 μm) evoked a robust firing of a POMC cell, which was also excited by kisspeptin. C, Time course of the effects of DHPG and kisspeptin on spike frequency for the POMC cell in trace in A. D, Bar graph showing the depolarization of POMC cells by DHPG (10–50 μm) and kisspeptin (1 μm). The membrane potential values under the traces in A and B indicate the membrane potentials before applying DHPG. Error bars indicate SEM. **p < 0.01, paired t test.