FIG. 1.

Treatment with ethanol and D3T increases the level of Nrf2 protein in mouse embryos. Western blot was performed to analyze the level of Nrf2 protein in GD 8 mouse embryos 6 h after exposure to vegetable oil or ethanol. Embryo lysates were prepared from whole embryos treated with vegetable oil (Control), 2.9 g/kg ethanol (EtOH), pretreated with 5 mg/kg D3T alone (D3T), or treated with both ethanol and D3T (EtOH+D3T). Data are expressed as fold change over control and represent the mean ± SEM of three separate experiments. *p < 0.05 vs. control. ♣ p < 0.05 vs. EtOH.