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D3T pretreatment significantly reduced ROS generation in mouse embryos exposed to ethanol in vivo. ROS generation was measured in GD 8 mouse embryos 6 h after exposure to vegetable oil (Control), 2.9 g/kg ethanol (EtOH), pretreatment with 5 mg/kg D3T alone (D3T), or treatment with both ethanol and D3T (EtOH+D3T). Data are expressed as relative fluorescence intensity units and represent the mean ± SEM of three separate experiments. *p < 0.05 vs. control. ♣ p < 0.05 vs. EtOH.
