FIG. 1.

HIV-specific CD4⁺ T cells produce TGF-β. PBMCs were stimulated with HIV peptides then stained with anti-TGF-β PE, anti-CD3 AmCyan, anti-CD4 PerCP CY5.5, and anti-CD8 PE CY7, and analyzed by flow cytometry. Samples were first gated on the CD3⁺/CD4⁺ lymphocyte population and then the percent of TGF-β-positive CD4⁺ T cells was determined. Representative plots of HIV-specific CD4⁺ T cells expressing TGF-β after subtraction of the background values.