FIG. 3.

TGF-β receptor II blockade increases IFN-γ expression by HIV-specific T cells. PBMCs were stimulated with Gag peptides in the presence of anti-TGF-β R II (or isotype control). PBMCs were then stained with anti-IFN-γ FITC, anti-CD3 AmCyan, anti-CD4 PerCP Cy5.5, and anti-CD8 PE Cy7 and analyzed by flow cytometry. Samples were first gated on the CD3⁺/CD4⁺ and CD3⁺/CD8⁺ lymphocyte populations and then the percent of IFN-γ-positive cells was determined after subtraction of the background values. Plots are from three independent experiments yielding similar results.