FIG. 6.

Analysis of the immunophenotypic profile of HIV-specific TGF-β-positive CD4⁺ T cells. PBMCs (n = 6) were stimulated with HIV peptides, then stained for regulatory markers and the percentage of TGF-β-positive CD4⁺ T cells was determined by flow cytometry. Samples were first gated on the CD3⁺/CD4⁺ lymphocyte population and then the percentages of TGF-β-positive cells were determined and the extent of FOX P3, CD25, and CD127 expression was also examined. Gating was performed using the fluorescence-minus-one (FMO) control for each marker. Representative plots of the phenotype of the HIV-specific TGF-β-positive CD4⁺ T cells expressing FOX P3, CD25, and CD127. The values marked with an asterisk represent the fraction of TGF-β-positive cells that expresses FOX P3, CD25, or CD127 over the total number of TGF-β-positive cells (equivalent to 100%).