The β-subunits of FSH and LH are present in LβT2 cells but not αT3-1 cells. LβT2 and αT3-1 cells were grown on glass coverslips, fixed, and immunostained. The proteins were visualized using antibodies specific to FSH (FSHβ), LH (LHβ), α-GSU, or steroidogenic factor 1 (SF-1) and a Texas red-conjugated antirabbit IgG secondary antibody. Nuclei were stained with 4,6-diamidino-2-phenylindole. Representative fields are shown.