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. Author manuscript; available in PMC: 2010 Sep 3.
Published in final edited form as: Science. 2006 Oct 13;314(5797):298–300. doi: 10.1126/science.1131000

Fig. 4.

Fig. 4

Inhibitory activity is mediated in part by autocrine TGFβ in cultured cells. (A) Confocal section of primary mammary epithelial tubule stained for TGFβ1, with graphs representing relative pixel intensity (arbitrary units, a.u.) as a function of distance along tubules (red) and away from tubules (green). Numerical predictions are superimposed as solid blue curves to fit the intensity range. Frequency maps 24 hours after induction of branching in tubules of (B) control cells and (C) cells overexpressing active TGFβ1 confirm that TGFβ1 inhibits branching. (D and E) Positional control of branching is disrupted by blocking signaling of endogenous TGFβ1. Shown are frequency maps 24 hours after induction of branching in tubules of (D) vector control cells and (E) cells overexpressing dominant negative TGFβ receptor type II (HA-DNTβRII). Scale bars, 50 μm.