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Overexpression of JNK2 results in elevated NF-AT transcriptional activity. (A) NF-AT activity assay of Jurkat T cells transfected with control vector and a JNK expression construct together with NF-AT luciferase reporter construct. Protein extracts were prepared 24 h after transfection, and luciferase activity was measured in duplicate. (B) JNK2 protein levels in Jurkat T cells transfected with control vector and a JNK expression construct were determined by Western analysis.
