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. 2010 Sep 3;5(9):e12558. doi: 10.1371/journal.pone.0012558

Figure 2. Recombinant DBL5-ε, DBL6-ε, and DBL5-6-ε domains induce antibodies that block adhesion to BeWo cells in flow binding assays.

Figure 2

(A) Schematic representation of the binding inhibition assay mimicking the flow in placental physiological conditions (B) Inhibition of FCR3 PEs adhesion. Purified IgG at 0.5 mg/ml from a group of 5 mice immunized either with Freund or Alum as adjuvants were tested for inhibition of binding of FCR3-PEs to BeWo cells in flow binding assays. The numbers of bound PEs in the presence of IgG from preimmune serum was used as a reference value. The numbers of bound PEs for anti DBL IgG were used to calculate the binding inhibition as a percentage relative to the reference value. (C) and (D) Recombinant domains from heterologous parasites induce transcending adhesion blocking activity. Purified IgG at 0.5 mg/ml from groups of 5 mice immunized with heterologous DBL5-ε and DBL5-6-ε domains were tested for inhibition of binding of FCR3-PEs to BeWo and ScC2 cells in flow binding assays. Amino acid sequence identity percentages between the heterologous DBL domain and the homologous FCR3 DBL are presented.