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. 2010 Sep 3;5(9):e12558. doi: 10.1371/journal.pone.0012558

Figure 3. Recombinant FCR3-DBL5-6-ε depletes the adhesion blocking activity in goat serum.

Figure 3

(A) Goat Immunization with FCR3-DBL5-6-ε induces adhesion blocking antibodies. Goat purified IgG was tested for inhibition of FCR3-binding of PEs to BeWo and ScC2 cells in flow binding assays after the first boost, second boost and four weeks after the second boost. (B) Recombinant FCR3-DBL5-6-ε depletes the adhesion blocking activity. Goat purified IgG obtained four weeks after the second boost (D98) was depleted on immobilized FCR3-DBL5-6-ε. The inhibition of FCR3-PEs adhesion of IgG before antigen depletion, the depleted IgG, and elution of anti DBL5-6-ε were tested in flow binding assays using BeWo and ScC2 cells.