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. 2010 Sep 3;5(9):e12564. doi: 10.1371/journal.pone.0012564

Figure 3. KB-L30 cells exhibit alterations in the expression of β-tubulin isotypes and mutations in the class III β-tubulin gene.

Figure 3

The expression of different β-tubulin isotypes in KB and KB-L30 cells was analyzed by RT-PCR. GAPDH was used as an internal control (A). RT-PCR and nested-PCR analyzes of exons 1, 2, 3 and 4 in the class III β-tubulin gene of KB and KB-L30 cells. First lane, KB cells and second lane, KB-L30 cells (B). PCR-SSCP analyzes of exons 1, 2, 3 and 4 in the class III β-tubulin gene of KB and KB-L30 cells. First lane, KB cells and second lane, KB-L30 cells (C).