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. 2010 Sep 3;5(9):e12564. doi: 10.1371/journal.pone.0012564

Figure 4. Mutations in exon 4 of βIII-tubulin interfere with the sensitivity of BPR0L075 in KB-L30 cells.

Figure 4

DNA sequencing of the βI-tubulin genomic DNA reveals six point mutations present in exon 4 (A). Translated amino-acid sequence of the mutated exon 4 of the βI-tubulin gene (B). The expression of the mutated βI-tubulin protein and its incorporation in the microtubule networks were revealed by immuno-fluorescent microscopy. Cells were probed with anti-βI-tubulin antibody (C). Transfection of the mutated βI-tubulin gene into KB cells reduced the drug sensitivity to BPR0L075 in vitro (D).