Figure 1. Arabidopsis plants expressing AtHKT1;1 within the root epidermal and cortical cells.

(A) Representative fluorescence stereomicroscope and (B) confocal laser microscope images of J1551 showing GFP fluorescence specifically within the root epidermal and cortical cells. Tissue was stained with propidium iodide. GFP and propidium iodide images were captured separately and overlaid to create the composite image. Tissues labelled include the epidermis (ep), cortex (c), endodermis (en) and xylem parenchyma (xp). (C) Transactivation of uidA in J1551 produces GUS staining specifically within the root epidermal and cortical cells [Scale bars  = 10 mm in (A), 75 µm in (B) and 75 µm in (C)]. (D) Concentration of Na⁺ within the leaves of T1 J1551 and J1551 UAS_GAL4:AtHKT1;1 plants grown on soil and watered with nutrient solution containing 2 mM NaCl (n = 12 for background and 45 independent events for AtHKT1;1, error bars represent SEM). (E) Accumulation of Na⁺ within the leaves of T2 J1551 and J1551 UAS_GAL4:AtHKT1;1 plants grown on soil and watered with nutrient solution containing 5 mM NaCl (n = 3 for background and 20 for AtHKT1;1 lines for each of 4 independent events, presented as a grand average, error bars represent SEM). Statistical significance from the J1551 line was determined using Student's t-test, *P<0.05.