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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: J Immunol. 2010 Aug 13;185(6):3740–3749. doi: 10.4049/jimmunol.1001231

FIGURE 7.

FIGURE 7

CD86 upregulation is cAMP, PKA, and PKC-dependent, but Ca2+-independent. BMDM were left non-treated or treated with 1 μg/ml LPS or 25 μg of MV with or without additional treatment of 10 μM intracellular calcium chelator BAPTA/AM in A, 50 μM PKC inhibitor Bisindolylmaleimide I HCl (BIM) in B, 10 μM adenylate cyclase inhibitor MDL-12330A in C, or 10 μM PKA inhibitor H-89 in D, for 18 hours. Surface CD86 MFI ± SEM of n=3 are shown.