Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Jul 17;40(9):683–689. doi: 10.1016/j.ibmb.2010.07.001

Figure 1. SDS-stable complex formation between HP6Xa and serpin-5 (A) or serpin-4 (B).

Figure 1

ProHP6Xa (40 ng) was activated by factor Xa and then incubated for 10 min at room temperature with serpin-5 or serpin-4 at a molar ratio of 1:1 or 10:1 (serpins : proHP6Xa). In control reactions, proHP6Xa or factor Xa were omitted. The samples were subjected to SDS-PAGE and immunoblot analysis using antiserum against HP6 (left panel) or serpin (right panel). Bands representing a serpin-HP6 complex are marked by arrow heads. HP6 antibody recognized the 39-kDa proHP6Xa zymogen and a 29-kDa catalytic domain produced upon activation by factor Xa. Size and positions of molecular mass standards are indicated to the left of each blot.