Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Jul 17;40(9):683–689. doi: 10.1016/j.ibmb.2010.07.001

Figure 2. Rate of formation of covalent complex between HP6 and serpin-4 or -5.

Figure 2

(A) Activated HP6Xa (40 ng) was incubated with serpin-4 (480 ng) or serpin-5 (460 ng) at room temperature for 0, 0.5, 1, 1.5, 2, 2.5 and 3 min. The reaction mixtures were separated by SDS-PAGE, followed by immunoblot analysis with antiserum against M. sexta HP6. The size and position of molecular weight standards are indicated on the left. (B) Quantitative analysis of 66-kDa band in (A) intensity by densitometry. Net intensity of the 66-kDa band corresponding to serpin-proteinase complex was analyzed using Carestream Molecular Imaging Software 5.0, and plotted against incubation time.