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. Author manuscript; available in PMC: 2011 Oct 13.
Published in final edited form as: Neuroscience. 2010 Jul 17;170(2):408–416. doi: 10.1016/j.neuroscience.2010.07.025

Fig. 6.

Fig. 6

Regulation of DAT transport activity and surface levels in syn 1A co-expressing cells. 6xHis-rDAT-LLCPK1 cells transiently transfected with vector or syn 1A cDNA were treated with vehicle or 1 μM PMA for 15 min and analyzed for [3H]DA transport activity or subjected to cell surface biotinylation. A. [3H]DA transport activity (means ± S.E. of 5 independent experiments performed in triplicate). *, p<0.05; **, p<0.01, ***, p<0.001 relative to control or indicated treatment by ANOVA. Upper panel, representative blot of total DAT levels. Quantification of blot intensities p>0.05 relative to control by ANOVA. B. Representative immunoblot and quantification of biotinylated DAT (means ± S.E. of 4 independent experiments performed in duplicate). *, p<0.05, **, p<0.01, relative to control by ANOVA.